Additional Information
| Product Name: | Ubiquitin/SUMO/Nedd8 E2 Screening Kit |
|---|---|
| Also Known As: | E2 screening kit |
| Catalog No.: | J1300 |
| Size: | 1 Kit |
| Molecular Weight: | N/A |
| Species: | Human |
| Source: | Bacterial or insect cell recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | 90% - 95% based on SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | N/A |
Details
Description
The ubiquitin/SUMO/Nedd8 E2 Screening Kit II (Cat# J1300) contains ubiquitin (Ub)-activating enzyme (UbE1), SUMO-activating enzyme (6xHis-SAE1/SAE2), Nedd8-activating enzyme (APPBP1/6xHis-UBA3), Ub, SUMO2, Nedd8, and 29 Ub-conjugating enzymes (E2s). It can be used for 1) identification of the E2 enzyme paired for a specific Ub ligase (E3) for a protein substrate, and 2) assessing/profiling E2 activity by monitoring the E2-Ub thioester conjugate formation under non-reducing condition.
Comparing to the Ubiquitin E2 Screening Kit (Cat# J1100), this kit contains additional SUMO or Nedd8-activating E1, SUMO2 and Nedd8 for determination of SUMO2 and Nedd8 conjugation.
Components
• 20X UBE1 (2µM) 100µl
• 20X 6xHis-SAE1/SAE2 (2µM, SUMO E1) 25µl
• 20X APPBP1/6xHis-UBA3) (2µM, Nedd8 E1) 25µl
• 10X E2 Ub-conjugating enzymes
(29 E2s, see table above, 20µM each) 20µl each
• 10X Human Ubiquitin (500µM) 200µl
• 10X SUMO2 (500µM) 25µl
• 10X 6XHis-Nedd8 (150 µM) 50µl
• 10X Ubiquitination Buffer 1ml
• 40mM ATP 250µl
E2 Name
1: 6XHis-UbE2A (Rad6A)
2: 6XHis-UbE2B
3: 6XHis-UbE2C(UbcH10 )
4: 6XHis-UbE2D1 (UbcH5A)
5: 6XHis-UbE2D2 (UbcH5B)
6: 6XHis-UbE2D3 (UbcH5C)
7: 6XHis-UbE2D4 (UbcH5D)
8: 6XHis-UbE2E1 (UbcH6)
9: 6XHis-UbE2E2 (UbcH8)
10: 6XHis-UbE2E3 (UbcH9)
11: 6XHis-UbE2F
12: 6XHis-UbE2G1
13: 6XHis-UbE2G2
14: 6XHis-UbE2H (UbcH2)
15: 6XHis-UbE2I
16: 6XHis-UbE2J1(1-282)
17: GST-UbE2K (E2-25K)
18: 6XHis-UbE2L3
19: 6XHis-UbE2L6
20: 6XHis-UbE2M (UbcH12)
21: 6XHis-UbEN (Ubc13/Uev1a)
22: 6XHis-UbE2Q2
23: 6XHis-UbE2R1 (Cdc34)
24: 6XHis-UbE2R2
25: 6XHis-UbE2S
26: 6XHis-UbE2T
27: 6xHis-Ubc13/UbE2V2
28: 6XHis-UbE2W
29: 6XHis-UbE2Z
