RELAYTR Ubiquitin E2 Profiling Kit

The RELAYTR Ubiquitin E2 Profiling Kit is based on the TR-FRET technology that offers a simple, sensitive, homogeneous, and stable readouts to measure the activity of ubiquitin-conjugating enzymes (E2) by determination of the E2-ubiquitin thioester conjugates.

23 ubiquitin-conjugating enzymes (E2s) are included in the kit. 50 x 20 µl reactions for each E2.


Implications

1) E2 profiling.

2) Hit/lead discovery and validation.


Additional Information

Product Name: RELAYTR Ubiquitin E2 Profiling Kit
Also Known As: TR-FRET ubiquitin E2 profiling kit
Catalog No.: T5000
Size: 50 x 20 µl / E2, 23 E2s
Molecular Weight: No
Species: Human
Source: Recombinant proteins
Stock: No
Concentration: See tube label
Quality Assurance: > 90% by SDS-PAGE; Validated by RELAYTR ubiquitination assays.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: No
Image(s): Images:
(Click image to enlarge)

A. Schematic of the RELAYTR E2-Ub Charging Assay using 6xHis-tagged E2, fluorescein-Anti-6xHIS antibody and terbium-Ub.
B. Representative kinetic curves and the FRET ratio (S520/S490) of the RELAYTR E2-Ub charging assay with ATP.
C. Signal-to-background ratios of E2-Ub charging assays for UbE2D4, UbE2E2 and Ubc13/UbE2V2. Data were shown as mean ± S.D. of four reactions.

Shipping Method: Dry ice shipping
References: 1. https://www.bmglabtech.com/en/tr-fret/

Details

The RELAYTR Ubiquitin E2 Profiling Kit is based on the TR-FRET technology that offers a simple, sensitive, homogeneous, and stable readouts to measure the activity of ubiquitin-conjugating enzymes (E2) by determination of the E2-ubiquitin thioester conjugates.
23 ubiquitin-conjugating enzymes (E2s) are included in the kit. 50 x 20 µl reactions for each E2.
Implications
1) E2 profiling.
2) Hit/lead discovery and validation.