Additional Information
| Product Name: | 6xHis-UbE2J1(1-282) | ||
|---|---|---|---|
| Also Known As: | UBC6; UBC6E; Ubc6p; CGI-76; NCUBE1; HSPC153; HSPC205; NCUBE-1; HSU93243 | ||
| Catalog No.: | C2601 | ||
| Size: | 100 µg | ||
| Molecular Weight: | 31.2 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~80% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | N/A | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Lester D, et al. (2000) Biochem Biophys Res Commun 269, 474 – 480. 2. Tiwari S, et al. (2001) J Biol Chem 276, 16193 – 16200. 3. Lenk U, et al. (2002) J Cell Sci 115(Pt 14), 3007 – 3014. 4. Wang X, et al. (2009) J Cell Biol 187(5), 655 – 668. |
Details
UbE2J1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. UBE2J1 is a membrane protein localized on the endoplasmic reticulum (ER), where it catalyzes ubiquitination of misfolded membrane proteins with other ubiquitination components. UbE2J1(1-282) lacks the C-terminal membrane-localization region, but still possesses Ub conjugating activity.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2J1(1-282) |
