RELAYTR ITCH Activity Assay Kit

RELAYTR ITCH Activity Assay Kit is based on the TR-FRET technology that offers a sensitive, homogeneous, stable, and high signal-to-background ratio assay to measure the ubiquitin ligase activity of ITCH via assessing ITCH autoubiquitination.

Implications

1) Screening inhibitors of ITCH.

2) Profiling ubiquitin ligases.

3) Assessing the effect of ITCH binding partners on its ubiquitin ligase activity.

Additional Information

Product Name: RELAYTR ITCH Activity Assay Kit
Also Known As: TR-FRET ITCH Ubiquitin Ligase Activity Assay Kit
Catalog No.: T4010
Size: 400 x 20 µl Reactions
Molecular Weight: No
Species: Human
Source: Recombinant proteins
Stock: No
Concentration: See tube label
Quality Assurance: > 90% by SDS-PAGE; Validated by RELAYTR ubiquitination assays.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: No
Image(s): Images:
(Click image to enlarge)

A. Schematic of RELAYTR Ubiquitin Ligase Activity Assay.
B. RS is the FRET ratio of positive reaction; RB is the FRET ratio of blank reaction (no E3 compared to positive reaction). Data (mean ± S.D. of four reactions) were obtained from 50 nM ITCH autoubiquitination using the kit. Time 0 is ~5 min after ATP addition.

Shipping Method: Dry ice shipping
References: 1. https://www.bmglabtech.com/en/tr-fret/

Details

RELAYTR ITCH Activity Assay Kit is based on the TR-FRET technology that offers a sensitive, homogeneous, stable, and high signal-to-background ratio assay to measure the ubiquitin ligase activity of ITCH via assessing ITCH autoubiquitination.
Implications
1) Screening inhibitors of ITCH.
2) Profiling ubiquitin ligases.
3) Assessing the effect of ITCH binding partners on its ubiquitin ligase activity.