Additional Information
| Product Name: | RELAYTR Ubiquitin Ligase Activity Assay Kit | ||
|---|---|---|---|
| Also Known As: | TR-FRET E3 Ubiquitin Ligase Activity Assay Kit | ||
| Catalog No.: | T4000 | ||
| Size: | 400 x 20 µl Reactions | ||
| Molecular Weight: | No | ||
| Species: | Human | ||
| Source: | Recombinant proteins | ||
| Stock: | No | ||
| Concentration: | See tube label | ||
| Quality Assurance: | > 90% by SDS-PAGE; Validated by RELAYTR ubiquitination assays. | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet | ||
| NCBI RefSeq: | No | ||
| Image(s): | Images:
(Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. https://www.bmglabtech.com/en/tr-fret/ |
Details
RELAYTR Ubiquitin Ligase Activity Assay Kit is based on the TR-FRET technology that offers a sensitive, homogeneous, stable, and high signal-to-background ratio assay to assess E3 ubiquitin ligase activity via measuring E3 autoubiquitination. The provided terbium-ubiquitin and fluorescein-ubiquitin mix can support various types of polyubiquitin chain formation as well as monoubiquitination, thereby enabling TR-FRET signaling.
This kit could be used to determine the ubiquitin ligase activity or screening compounds for any given E3s that are either polyubiquitinated or multi-monoubiquitinated.
The ubiquitin ligase CHIP is included in the kit as a positive control.
This kit could be used to determine the ubiquitin ligase activity or screening compounds for any given E3s that are either polyubiquitinated or multi-monoubiquitinated.
The ubiquitin ligase CHIP is included in the kit as a positive control.
