RELAYTR UbE3A Activity Assay Kit

RELAYTR UbE3A Activity Assay Kit is based on the TR-FRET technology that offers a sensitive, homogeneous, stable, and high signal-to-background ratio assay to measure the ubiquitin ligase activity of UbE3A via assessing UbE3A autoubiquitination.

Implications

1) Screening inhibitors of UbE3A.

2) Profiling ubiquitin ligases.

3) Assessing the effect of UbE3A binding partners on its ubiquitin ligase activity.

Additional Information

Product Name: RELAYTR UbE3A Activity Assay Kit
Also Known As: TR-FRET UbE3A Ubiquitin Ligase Activity Assay Kit
Catalog No.: T4020
Size: 400 x 20 µl Reactions
Molecular Weight: No
Species: Human
Source: Recombinant proteins
Stock: No
Concentration: See tube label
Quality Assurance: > 90% by SDS-PAGE; Validated by RELAYTR ubiquitination assays.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: No
Image(s): Images:
(Click image to enlarge)

A. Schematic of RELAYTR Ubiquitin Ligase Activity Assay.
B. RS is the FRET ratio of positive reaction; RB is the FRET ratio of blank reaction (no E3 compared to positive reaction). Data (mean ± S.D. of four reactions) were obtained from 12.5 nM GST-UbE3A autoubiquitination using the kit. Time 0 is ~5 min after ATP addition..

Shipping Method: Dry ice shipping
References: 1. https://www.bmglabtech.com/en/tr-fret/

Details

RELAYTR UbE3A Activity Assay Kit is based on the TR-FRET technology that offers a sensitive, homogeneous, stable, and high signal-to-background ratio assay to measure the ubiquitin ligase activity of UbE3A via assessing UbE3A autoubiquitination. Implications
1) Screening inhibitors of UbE3A.
2) Profiling ubiquitin ligases.
3) Assessing the effect of UbE3A binding partners on its ubiquitin ligase activity.