Additional Information
| Product Name: | UBE1 Rabbit pAb |
|---|---|
| Catalog No.: | Y3062-100 |
| Also Known As: | UBA1; A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1; POC20; SMAX2; UBA1A; UBE1; UBE1X |
| Size: | 100 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBE1. |
| Swiss Prot. #: | P22314 |
| Calculated MW: | 118 kDa |
| Detected MW: | 121 kDa |
| Applications: | WB (1:500 - 1:2,000) IHC (1:50 - 1:200) IF (1:50 - 1:200) IP (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Handley PM, et al. (1991) Proc Nat Acad Sci USA 88, 258 - 262. 2. Jin J, et al. (2007) Nature 447, 1135 - 1138. 3. Zheng M, et al. (2009) Mol Biol Rep 37, 1413 - 1419. |
Details
UBE1 activiates ubiquitin, acting as the first step in the E1/E2/E3 cascade-mediated protein ubiquitination. UBE1 activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. E1 activated Ub is then transferred to an E2 enzyme.
