Additional Information
| Product Name: | SAE1 Rabbit pAb |
|---|---|
| Catalog No.: | Y3052-100 |
| Also Known As: | SAE1; AOS1; HSPC140; SUA1; UBLE1A |
| Size: | 100 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human SAE1 (NP_005491.1) |
| Swiss Prot. #: | Q9UBE0 |
| Calculated MW: | 38 kDa |
| Detected MW: | 38 kDa |
| Applications: | WB (1:500 - 1:2,000) IP (not tested) IHC (not tested) IF (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Desterro JMP, et al. (1999) J Biol Chem 274, 10618 - 10624. 2. Azuma Y, et al. (2001) FASEB J 15, 1825 - 1827. 3. Lois LM and Lima CD (2005) EMBO J 24, 439 - 451. |
Details
SAE1 is a subunit of the heterodimeric SUMO activating enzyme consisting of SAE1 and SAE2. Matured SUMO is conjugated onto the catalytic cysteine residue of SAE2 by formation of a thioester bond in an ATP-dependent reaction. The activated SUMO is then passed on to UbE2I (an E2) to be attached to a substrate.
