Additional Information
| Product Name: | NAE1 Rabbit pAb |
|---|---|
| Catalog No.: | Y3002-100 |
| Also Known As: | NAE1; A-116A10.1; APPBP1; HPP1; ula-1 |
| Size: | 100 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NAE1 (NP_003896.1) |
| Swiss Prot. #: | Q13564 |
| Calculated MW: | 60 kDa |
| Detected MW: | 60 kDa |
| Applications: | WB (1:1000 - 1:4,000) IP (not tested) IHC (not tested) IF (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Gong L and Yeh ETH, (1999) J Biol Chem 274, 12036 - 12042. 2. Bohnsack RN and Haas AL, (2003) J Biol Chem 278, 26823 - 26830. |
Details
NAE1 (APPBP1) is a subunit of the heterodimeric Nedd8 activating enzyme consisting of NAE1 and UBA3. Nedd8 is conjugated onto the catalytic cysteine residue of UBA3 by formation of a thioester bond in an ATP-dependent reaction. The activated Nedd8 is then passed on to UbE2M (an E2) to be attached to a substrate. Nedd8 is mainly conjugated on Cullins to increase the activity of the Cullin-RING E3 Ub ligases.
