Additional Information
| Product Name: | ITCH Rabbit pAb [KO Validated] |
|---|---|
| Catalog No.: | Y5832-100 |
| Also Known As: | ITCH; ADMFD; AIF4; AIP4; NAPP1 |
| Size: | 100 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ITCH (NP_001244066.1). |
| Swiss Prot. #: | Q96J02 |
| Calculated MW: | 103 kDa |
| Detected MW: | 103 kDa |
| Applications: | WB (1:500 - 1:2,000) IHC (1:50 - 1:200) IP (not tested) IF (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Wood JD et al. (1998) Mol Cell Neurosci 11, 149 - 160. 2. Courbard JR, (2002) J Biol Chem 277, 45267 - 45275. |
Details
ITCH is a HECT domain-containing E3 ubiquitin ligase. HECT domain E3 ubiquitin ligases accept ubiquitin from E2 ubiquitin-conjugating enzymes, and then transfer ubiquitin to protein substrates. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses.
