Ub-Rhodamine 110

Additional Information

Product Name:

Ub-Rhodamine 110

Also Known As:

N/A

Catalog No.:

M3020

Size:

50 µg

Molecular Weight:

8936 Da by MS

Species:

Human

Source:

Synthetic

Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

Concentration:

N/A

Quality Assurance:

≥95% by RP-HPLC and SDS-PAGE

Storage:

Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.

PDF Data Sheet:

PDF Datasheet， MSDS

NCBI RefSeq:

N/A

Image(s):

(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: Ub-Rhodamine 110

Activity assay of Ub-Rhodamine 110 (100 nM) with varying concentrations UCH-L3 (5 - 313 pM).

Reaction buffer: 20 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 2 mM DTT. Reactions contained 0.5 μM Ub-Rhodamine 110 alone (control, brown line), or with 10 nM GST-Usp15 (gray line) or with 20 nM GST-Usp15 (yellow line). Rhodamine110 fluorescence was monitored by using a plate reader with excitation/emission filters at 485/20 and 530/30 nm, respectively.

Shipping Method:

Dry ice shipping

References:

1. A. Tirat et al., (2005) Anal. Biochem., 343, 244-255.
2. U. Hassiepin et al., (2007) Anal. Biochem., 371, 201-207.

Details

Ub-Rhodamine 110 is a quenched, fluorescent substrate for deubiquitinating enzymes. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110 results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).

Sample Preparation:
Dilute stock solution into required buffer. Typical reaction concentrations vary from 0.1 - 1 µM

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: Ub-Rhodamine 110

Activity assay of Ub-Rhodamine 110 (100 nM) with varying concentrations UCH-L3 (5 - 313 pM).