Additional Information
| Product Name: | 6xHis-NEMO (UBAN) | ||
|---|---|---|---|
| Also Known As: | IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma | ||
| Catalog No.: | I1520 | ||
| Size: | 100 µg | ||
| Molecular Weight: | 18.4 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_001099857 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. van Wijk SJ, et al. (2013) Nat Protoc. 8(7):1449-58 2. van Wijk SJ, et al. (2012) Mol Cell. 14;47(5):797-809 |
Details
6xHis-NEMO (UBAN) was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO encompassing amino acids 183-339. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. 6xHis-NEMO (UBAN) can be precipitated using Nickel resin. After washing, 6xHis-NEMO (UBAN) and its bound proteins can be eluted by a buffer containing 200 mM imidazole.
Images:
(Click image to enlarge)
Note:
1. NEMO(183-339) may also bind long polyubiquitin chains linked via lysine 63.
2. Dissolve 10 mM glutathione in a neutral buffer could drop pH to 3-4. Use 0.2 M NaOH to ad-just pH back to neutral.
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-NEMO (UBAN) |
Note:
1. NEMO(183-339) may also bind long polyubiquitin chains linked via lysine 63.
2. Dissolve 10 mM glutathione in a neutral buffer could drop pH to 3-4. Use 0.2 M NaOH to ad-just pH back to neutral.
