Additional Information
| Product Name: | RELAYTR Ubiquitination Buffer (10X) |
|---|---|
| Also Known As: | TR-FRET ubiquitination buffer |
| Catalog No.: | T1001-5 |
| Size: | 5 ml |
| Molecular Weight: | No |
| Species: | No |
| Source: | No |
| Stock: | No |
| Concentration: | 10X. Containing HEPES, NaCl, MgCl2, TCEP, TWEEN-20 |
| Quality Assurance: | Validated by various RELAYTR ubiquitination assays. |
| Storage: | Store at -20°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet， |
| NCBI RefSeq: | No |
| Image(s): | No |
| Shipping Method: | Wet ice shipping |
| References: | No |
Details
This buffer is optimized to keep UbE1, E2 ubiquitin-conjugating enzymes and E3 ubiquitin ligases stable under room termperature for up to 4 hours. MgCl2 is included in the buffer. Addition of ATP (Catalog # T1501) is required for activation of UbE1 to initiate a ubiquitination reaction. Reactions with all components but ATP can serve as the negative reaction (background).
This buffer can be used as the reaction buffer as well as the component dilution buffer.
