RELAYTR NEDD4 Activity Assay Kit

RELAYTR NEDD4 Activity Assay Kit utilizes the TR-FRET technology to measure the ubiquitin ligase activity of NEDD4 by detecting its autoubiquitination (see schematic below). The kit is easy to use and sensitive with high signal-to-background ratio readout.


Implications

1) Screening inhibitors/activators of NEDD4.

2) Profiling ubiquitin ligases.

3) Assessing the ubiquitin ligase activity of NEDD4.



Additional Information

Product Name: RELAYTR NEDD4 Activity Assay Kit
Also Known As: TR-FRET NEDD4 Ubiquitin Ligase Activity Assay Kit
Catalog No.: T4040
Size: 400 x 20 µl Reactions
Molecular Weight: No
Species: Human
Source: Recombinant proteins
Stock: No
Concentration: See tube label
Quality Assurance: > 90% by SDS-PAGE; Validated by RELAYTR ubiquitination assays.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: No
Image(s): Images:
(Click image to enlarge)

A. Schematic of RELAYTR Ubiquitin Ligase Activity Assay.
B.RS is the FRET ratio of positive reaction; RB is the FRET ratio of blank reaction (no E3 compared to positive reaction). Data (mean ± S.D. of four reactions) were obtained from 25 nM GST-NEDD4 autoubiquitination using the kit. Time 0 is ~5 min after ATP addition.

Shipping Method: Dry ice shipping
References: 1. https://www.bmglabtech.com/en/tr-fret/

Details

RELAYTR NEDD4 Activity Assay Kit utilizes the TR-FRET technology to measure the ubiquitin ligase activity of NEDD4 by detecting its autoubiquitination (see schematic below). The kit is easy to use and sensitive with high signal-to-background ratio readout.
Implications
1) Screening inhibitors/activators of NEDD4.
2) Profiling ubiquitin ligases.
3) Assessing the ubiquitin ligase activity of NEDD4.