Additional Information
| Product Name: | RELAYTR K48 Linkage-specific Deubiquitinase Assay Kit | ||
|---|---|---|---|
| Also Known As: | TR-FRET DUB Assay Kit | ||
| Catalog No.: | T5210 | ||
| Size: | 200 x 20 µl Reactions | ||
| Molecular Weight: | No | ||
| Species: | Human proteins, mouse monoclonal antibodies, | ||
| Source: | Recombinant proteins from E. coli | ||
| Stock: | Variable buffers | ||
| Concentration: | See tube label | ||
| Quality Assurance: | Validated by RELAYTR deubiquitination assay. | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet | ||
| NCBI RefSeq: | No | ||
| Image(s): | Images:
(Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. https://www.bmglabtech.com/en/tr-fret/ |
Details
RELAYTR K48 Linkage-specific Deubiquitinase Assay Kit I is based on the TR-FRET technology that offers a simple, sensitive, homogeneous, and stable assay to measure the activity of deubiquitinases (DUBs) that cleave K48-linked polyubiquitin chains. Each ubiquitin moiety in K48-linked diubiquitin (diUb) contains an HA tag or a DYKDDDDK tag, generating a robust FRET signal when binding with fluorescein-labeled anti-HA and terbium-labeled anti-DYKDDDDK antibodies. The mix of each individual ubiquitin is included as a negative control. The assay is high throughput compatible with a Z’ value > 0.87.
Implications
1) DUB activity or profiling assay.
2) Hit/lead compound discovery and validation.
Implications
1) DUB activity or profiling assay.
2) Hit/lead compound discovery and validation.
