6xHIS-RNF114

RNF114 is a RING-type E3 ubiquitin ligase that regulates cell cycle progression, immune responses, and DNA damage repair. Serval substrates have been identified, including cyclin-dependent kinase inhibitors (such as p21, p27, and p57), A20 and PARP1. It is a binding target for small molecules (like nimbolide) with potentials in targeted protein degradation drug development.

Additional Information

Product Name:

6xHIS-RNF114

Also Known As:

RING finger protein 114, Zinc finger protein 228, Zinc finger protein 313

Catalog No.:

K2030

Size:

50 µg

Molecular Weight:

28 kDa (with HIS tag)

Species:

Human

Source:

Bacterial recombinant

Stock:

20 mM Tris, pH7.6 at 4 ⁰C, 150 mM NaCl, 2 mM βME, 10% Glycerol.

Concentration:

See tube label

Quality Assurance:

~85% purity based on SDS-PAGE. Validated E3 ubiquitin ligase activity.

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet; MSDS

NCBI RefSeq:

Image(s):

(Click image to enlarge)

A. 3 ug 6xHIS-RNF114 separated in a 4-12% Bis-Tris gel (Coomassie-stained).
B. 6xHIS-RNF114 catalyzed polyubiquitin chain synthesis. The reaction containe 50 nM UbE1, 2 µM UbE2D4, 1 µM 6xHIS-RNF114, and 50 µM ubiquitin. Proteins wer separated in a 4-12% Bis-Tris gel and Coomassie-stained. Polyubiquitin chains (marked with asterisks) were synthesized. RNF114 autoubiquitination was not evident in Coomassie staining.

Shipping Method:

Dry ice shipping

References:

1. Middleton A. J. and Day C. L., (2025) The EMBO Journal, 44, 5921-23.

6xHIS-RNF114

Additional Information

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