6xHIS-MDM2

MDM2 (Mouse Double Minute 2) is a RING-type E3 ubiquitin ligase that serves as the primary negative regulator of the p53 tumor suppressor protein. It directly binds to the transactivation domain of p53 and promotes p53 polyubiquitination and proteasomal degradation. MDM2 plays a central role in controlling cell cycle progression, DNA repair, and apoptosis.

Additional Information

Product Name:

6xHIS-MDM2

Also Known As:

Human Double Minute 2, HDM2

Catalog No.:

K2020

Size:

50 µg

Molecular Weight:

57.5 kDa (with HIS tag)

Species:

Human

Source:

Bacterial recombinant

Stock:

20 mM Tris, pH7.6 at 4 ⁰C, 150 mM NaCl, 2 mM βME, 10% Glycerol.

Concentration:

See tube label

Quality Assurance:

~85% purity based on SDS-PAGE. Validated E3 ubiquitin ligase activity.

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet; MSDS

NCBI RefSeq:

Image(s):

(Click image to enlarge)

A. 3 ug 6xHIS-MDM2 separated in a 4-12% Bis-Tris gel (Coomassie-stained).
B. 6xHIS-MDM2 autoubiquitination. The reaction contained 50 nM UbE1, 2 µM UbE2D4, 1 µM 6xHIS-MDM2, and 50 µM ubiquitin. Proteins wer separated in a 4-12% Bis-Tris gel, visualized by Coomassie-staining.

Shipping Method:

Dry ice shipping

References:

1. Moll U.M., Petrenki O., (2003) Mol Cancer Res. 1(14), 1001-8.

6xHIS-MDM2

Additional Information

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