Additional Information
| Product Name: | SHARPIN Rabbit pAb |
|---|---|
| Catalog No.: | Y6212-100 |
| Also Known As: | SHARPIN; SIPL1; sharpin |
| Size: | 100 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human SHARPIN (NP_112236.3). |
| Swiss Prot. #: | Q9H0F6 |
| Calculated MW: | 39 kDa |
| Detected MW: | 39 kDa |
| Applications: | WB (1:500 - 1:2,000) IP (not tested) IHC (not tested) IF (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Daigo Y, et al. (2003) J Gastroent Hepatol 18, 712 - 718. 2. Tokunaga F, et al. (2011) Nature 471, 633 - 636. 3. Grabbe C and Dikic I, (2009) Chem Rev 109, 1481 - 1494. |
Details
SHARPIN is a component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. Sharpin harbors multiple functional motifs including an amino terminal coiled-coil (CC) domain, which has been shown to mediate the interaction between sharpin and the scaffold protein shank. The other two domains, ubiquitin-like domain (UBL) and NPL4 zinc finger domain (NZF), facilitate ubiquitin-mediated protein recognition and degradation.
