Additional Information
| Product Name: | Rpn2 Rabbit pAb |
|---|---|
| Catalog No.: | Y2312-20 |
| Also Known As: | PSMD1; P112; Rpn2; S1 |
| Size: | 20 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 794-953 of human Rpn2. |
| Swiss Prot. #: | Q99460 |
| Calculated MW: | 105 kDa |
| Detected MW: | 105 kDa |
| Applications: | WB (1:500 - 1:2,000) IP (not tested) IHC (not tested) IF (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1.Yokota K, et al. (1996) Mol Biol Cell 7, 853 - 870. 2. Ryu H, et al. (2014) Cell Rep 7, 1842 - 1848. |
Details
Rpn2 is the largest subunit of the 19S regulatory paticle (PA700) of the 26S proteasome. It recruits the ubiquitin receptor Adrm1 (Rpn13) to the 26S proteasome. The 26S proteaosme is composed of 33 different proteins and responsible for degrading polyubiquitinated proteins in a ATP hydrolysis-dependent manner. The 19S regulatory particle can bind polyubiqutin chains to recruit substrates, deubiquitinate to recycle ubiquitin, unfold substrate proteins under ATP hydrolysis, and translocate unfolded proteins to the 20S proteasome for hydrolysis.
