Additional Information
| Product Name: | Rpn1 Rabbit pAb |
|---|---|
| Catalog No.: | Y2302-100 |
| Also Known As: | PSMD2; P97; RPN1; S2; TRAP2 |
| Size: | 100 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human Rpn1. |
| Swiss Prot. #: | Q13200 |
| Calculated MW: | 100 kDa |
| Detected MW: | 108 kDa |
| Applications: | WB (1:500 - 1:2,000) IHC (1:50 - 1:200) IF (1:50 - 1:200) IP (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Boldin MP, et al. (1995) FEBS Lett 367, 39 - 44. 2. Tsurumi C, et al. (1996) Europ J Biochem 239, 912 - 921. |
Details
Rpn1 is the second largest subunit of the 19S regulatory paticle (PA700) of the 26S proteasome. It's one of the ubiquitin receptors on the proteasome that recurit polyubiquitinated proteins. The 26S proteaosme is composed of 33 different proteins and responsible for degrading polyubiquitinated proteins in a ATP hydrolysis-dependent manner. The 19S regulatory particle can bind polyubiqutin chains to recruit substrates, deubiquitinate to recycle ubiquitin, unfold substrate proteins under ATP hydrolysis, and translocate unfolded proteins to the 20S proteasome for hydrolysis.
