Additional Information
| Product Name: | Phospho-MDM2-S166 Rabbit pAb |
|---|---|
| Catalog No.: | Y8002-100 |
| Also Known As: | MDM2; ACTFS; HDMX; hdm2 |
| Size: | 100 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthetic phosphorylated peptide surrounding S166 of human MDM2 (NP_002383.2). |
| Swiss Prot. #: | Q00987 |
| Calculated MW: | 55 kDa |
| Detected MW: | 95 kDa |
| Applications: | WB (1:500 - 1:1 ,000) IF (1:50 - 1:100) IP (not tested) IHC (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Haupt Y, et al. (1997) Nature 387, 296 - 299. 2. Sasaki M, et al. (2011) Nature Med 17, 944 - 951. |
Details
MDM2 is an E3 ubiquitin ligase that targets the p53 protein for proteasomal degradation. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53.
