Additional Information
| Product Name: | Fluorescein-Anti-DYKDDDDK Mouse mAb |
|---|---|
| Also Known As: | Fluorescein-Anti-DYKDDDDK Antibody; FL-Anti-DYKDDDDK |
| Catalog No.: | T6040-50 |
| Size: | 50 µg |
| Molecular Weight: | 150 kDa |
| Species: | Mouse |
| Source: | No |
| Stock: | 10 mM PBS (pH 7.2), 10% glycerol, 0.09% sodium azide |
| Concentration: | See tube label |
| Quality Assurance: | Validated by immunoblotting assays |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet |
| NCBI RefSeq: | No |
| Image(s): | No |
| Shipping Method: | Wet ice shipping |
| References: | 1. https://www.bmglabtech.com/en/tr-fret/ |
Details
Fluorescein (6-FAM) is covalently conjugated on anti-DYKDDDDK mouse mAb (clone #: FG4R) and purified by gel filtration. Usually, the molar ratio of fluorescein to antibody is at the range of 3-10.
In TR-FRET assays, a typical concentration of fluorescein labeled antibody is at 0.8 - 1.2-fold of the concentration of the target protein. User should optimize the assay to achieve a desirable signal-to-background ratio, including the concentration and ratio of fluorescein or terbium labeled antibody, IgG or proteins corresponding to your specific assay design.
In TR-FRET assays, a typical concentration of fluorescein labeled antibody is at 0.8 - 1.2-fold of the concentration of the target protein. User should optimize the assay to achieve a desirable signal-to-background ratio, including the concentration and ratio of fluorescein or terbium labeled antibody, IgG or proteins corresponding to your specific assay design.
