Additional Information
| Product Name: | Fluorescein-Anti-6XHIS Mouse mAb | ||
|---|---|---|---|
| Also Known As: | Fluorescein-Anti-His Antibody | ||
| Catalog No.: | T6010-50 | ||
| Size: | 50 µg | ||
| Molecular Weight: | 150 kDa | ||
| Species: | Mouse | ||
| Source: | No | ||
| Stock: | 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) | ||
| Concentration: | See tube label | ||
| Quality Assurance: | Validated by RELAYTR E2-Ub charging assays. | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet | ||
| NCBI RefSeq: | No | ||
| Image(s): | Images:
(Click image to enlarge)
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| Shipping Method: | Wet ice shipping | ||
| References: | 1. https://www.bmglabtech.com/en/tr-fret/ |
Details
Fluorescein (6-FAM) is covalently conjugated on anti-His mouse mAb (clone: HIS.H8), and purified by gel filtration. Usually, the molar ratio of fluorescein to antibody is at the range of 3-12.
In TR-FRET assays, a typical concentration of fluorescein labeled antibody is equal or less than the concentration of the target protein. User should optimize the assay to achieve a desirable signal-to-background ratio, including the concentration and ratio of fluorescein or terbium labeled antibody, IgG or proteins corresponding to your specific assays.
TR-FRET assay reaction time is usually 1-3 hours in kinetic or end point assay.
In TR-FRET assays, a typical concentration of fluorescein labeled antibody is equal or less than the concentration of the target protein. User should optimize the assay to achieve a desirable signal-to-background ratio, including the concentration and ratio of fluorescein or terbium labeled antibody, IgG or proteins corresponding to your specific assays.
TR-FRET assay reaction time is usually 1-3 hours in kinetic or end point assay.
