Additional Information
| Product Name: | GST-HRV 3C Protease | ||
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| Also Known As: | N/A | ||
| Catalog No.: | P1002-10000 | ||
| Size: | 10 X 1 mg | ||
| Molecular Weight: | N/A | ||
| Species: | human rhinovirus (HRV) type 14 | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~ 90% by SDS-PAGE | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | N/A | ||
| Image(s): | Images:
(Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | No |
Details
Human rhinovirus 3C protease (HRV 3C Protease) is a cysteine protease that recognizes the Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro sequence (also called the PreScission site), and cleaves between Gln and Gly. The recombinant GST-HRV 3C can be used to cleave the GST tag in fusion proteins expressed using the pGEX-6p vector or other vectors, then removed by glutathione resin. It exhibits high specificity and activity at 4 0C in an overnight incubation reaction containing 1 mass unit of HRR 3C and 100 mass units of targeting protein.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified GST-HRV 3C Protease |
