TAMRA-Ubiquitin

TAMRA-Ubiquitin is a fluorescent ubiquitin, in which TAMRA (5-tetramethylrhodamine) is covalently conjugated onto the N-terminal region of human ubiquitin. All seven lysine residues and the N-terminus methionine of ubiquitin are available for ubiquitination. This product can be used for determination of protein ubiquitination or E1/E2/E3 enzyme activity using sensitive in-gel TAMRA fluorescence that can be detected using excitation/emission wavelengths at 550 nm/590 nm, respectively. Typically, 20 ng TAMRA-Ubiquitin is sufficient for in-gel visualization of TAMRA fluorescence.

Additional Information

Product Name:

TAMRA-Ubiquitin

Also Known As:

TAMRA-Ub, N-terminal TAMRA-Ub

Catalog No.:

E1161

Size:

1 mg

Molecular Weight:

9.1 KDa

Species:

Human

Source:

Chemical synthesis

Stock:

20 mM Tris, pH 7.6 at 4 °C, 150 mM NaC and 10% glycerol

Concentration:

See tube label

Quality Assurance:

>90% by SDS-PAGE

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet， MSDS

NCBI RefSeq:

N/A

Image(s):

Images:
(Click image to enlarge)

4ug TAMRA-Ub resolved in a 12% Bis-Tris gel

Shipping Method:

Dry ice shipping

References:

TAMRA-Ubiquitin

Additional Information

Details