Bovine 20S proteasome

The 20S proteasome has a barrel – shaped structure arranged as four heptomeric rings of αββα. In eukaryotes, each of α and β ring is composed of seven different proteins. The β1, β2 and β5 subunits have ‘caspase-like’, ‘trypsin-like’ and ‘chymotypsin-like’ activities, respectively. In 26S proteasome-mediated protein degradation, to entry the β chamber of the 20S proteasome that houses the proteolytic sites, a substrate protein has to pass through a substrate translocation channel consisting of the double-ring formed by six ATPases of PA700 and the α chamber formed by α subunits of the 20S proteasome.

Additional Information

Product Name:

Also Known As:

20S proteasome

Catalog No.:

A1401

Size:

50 µg

Molecular Weight:

700 kDa

Species:

Bovine

Source:

Bovine red blood cells

Stock:

20 mM Tris, 20 mM NaCl, 1 mM EDTA, 5 mM βME, 10% Glycerol

Concentration:

See tube label

Quality Assurance:

~95% by native-PAGE, see datasheet for gel image

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet， MSDS

NCBI RefSeq:

N/A

Image(s):


Coomassie-stained native-PAGE Lane 1: 5 µg purified Bovine 20S proteasome	Coomassie-stained native-PAGE Lane 1: 5 µg purified Bovine 20S proteasome


Activation of 5 nM 20S proteasome by 25 nM PA700 (Cat. # A1300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.	Activation of 5 nM 20S proteasome by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.	Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Shipping Method:

Dry ice shipping

References:

1. Waxman L, et al. (1987) J Biol Chem 262(6), 2451 – 2457.
2. Ganoth D, et al. (1988) J Biol Chem 263(25), 12412 – 12419.
3. Coux O, et al. (1996) Annu Rev Biochem 65, 801 – 847.
4. Kim HM, et al. (2011) Biochimica Biophysica Acta 1809(2), 67 – 79.

Details


Coomassie-stained native-PAGE Lane 1: 5 µg purified Bovine 20S proteasome	Coomassie-stained native-PAGE Lane 1: 5 µg purified Bovine 20S proteasome


Activation of 5 nM 20S proteasome by 25 nM PA700 (Cat. # A1300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.	Activation of 5 nM 20S proteasome by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.	Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.