Product Image(s)
[Cat.# A1400, A1401] Bovine 20S proteasome
| Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified Bovine 20S proteasome |
| Coomassie-stained native-PAGE Lane 1: 5 µg purified Bovine 20S proteasome |
| Activation of 5 nM 20S proteasome by 25 nM PA700 (Cat. # A1300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. |
| Activation of 5 nM 20S proteasome by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. |
| Activation of 5 nM 20S proteasome by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively. |
