Additional Information
| Product Name: | Aprotinin |
|---|---|
| Also Known As: | BPTI, Bovine pancreatic trypsin inhibitor |
| Catalog No.: | P1053-100 |
| Size: | 100 mg |
| CAS: | 9087-70-1 |
| Formula: | C284H432N84O79S7 |
| Molecular Weight: | 6511.5 g/mol |
| Form: | Powder |
| Quality Assurance: | ≥3.0 EPU/mg |
| Source: | Bovine lung |
| Storage: | Eligible for room temperature shipping. Store at -20°C upon receiving |
| PDF Data Sheet: | Download PDF datasheet, MSDS |
| Image(s): | N/A |
| Shipping Method: | Room temperature shipping |
| References: | 1. Fritz H., et al., Arzneimittelforschung, 1983, 33, 479. |
Details
Aprotinin is a 58 amino acid small protein derived from bovine lung tissue that is highly positively charged and contains three disulfide bonds. It is a competitive inhibitor of several serine proteases, including trypsin, chymotrypsin, thrombin, and plasmin. In clinical, it is used as an anti-clotting agent for reducing bleeding. This product is for research use only. Typical working concentration is 2 µg/ml.
Protease Inhibitor Stock Solution Preparation:
100X or 1000X stock solution preparation (after fully dissolved, aliquot to small volume, and store at -20 0C). - Aprotinin (catalog # P1053): Dissolve 20 mg in 10 ml of water or PBS to get 2 mg/ml stock (1000X). Working concentration is 2 µg/ml. - Leupeptin Hemisulfate (catalog # P1057): Dissolve 10 mg in 2.1 ml of DMSO or water to get 10 mM stock (1000X). Working concentration is 10 µM. - Pepstatin A (catalog # P1054): Dissolve 5 mg in 7.3 ml of DMSO to get 1 mM stock (1000X). Working concentration is 1 µM. - AEBSF HCl (catalog # P1056): Dissolve 10 mg in 0.416 ml of DMSO to get 100 mM stock (100X). Working concentration is 1mM. - Bestatin (catalog # P1055): Dissolve 5 mg in 16.2 ml DMSO to get 1 mM stock (1000X). Working concentration is 1 µM. - E-64D (catalog # P1051): Dissolve 5 mg in 1.4 ml DMSO to get 10 mM stock (1000X). Working concentration is 10 µM.
Protease Inhibitor Stock Solution Preparation:
100X or 1000X stock solution preparation (after fully dissolved, aliquot to small volume, and store at -20 0C). - Aprotinin (catalog # P1053): Dissolve 20 mg in 10 ml of water or PBS to get 2 mg/ml stock (1000X). Working concentration is 2 µg/ml. - Leupeptin Hemisulfate (catalog # P1057): Dissolve 10 mg in 2.1 ml of DMSO or water to get 10 mM stock (1000X). Working concentration is 10 µM. - Pepstatin A (catalog # P1054): Dissolve 5 mg in 7.3 ml of DMSO to get 1 mM stock (1000X). Working concentration is 1 µM. - AEBSF HCl (catalog # P1056): Dissolve 10 mg in 0.416 ml of DMSO to get 100 mM stock (100X). Working concentration is 1mM. - Bestatin (catalog # P1055): Dissolve 5 mg in 16.2 ml DMSO to get 1 mM stock (1000X). Working concentration is 1 µM. - E-64D (catalog # P1051): Dissolve 5 mg in 1.4 ml DMSO to get 10 mM stock (1000X). Working concentration is 10 µM.
