Products
- RELAYTR FRET Reagents & Kits
- Proteasomes / Activators / Subunits
- E1 Activating Enzymes
- E2 Conjugating Enzymes
- E3 Ubiquitin Ligases
- Polyubiquitin / PolySUMO Chains
- Ubiquitin / Ubiquitin-like Proteins / Mutants
- Deubiquitinating Enzymes
- Inhibitors
- Fluorescent Peptide / Protein Substrates
- Ubiquitin Binding Proteins
- Ubiquitin / UBL Derivatives
- Apoptosis / Necrosis
- Antibodies
- Kits
- Protein Expression / Purification Reagents
- DNA / Protein Ladders
- Covid-19 Research
Custom Services
Supported with extensive technical expertise and hands-on experience.
Protein Production
Recombinant protein expression in E. coli, insect, or mammalian systems, followed by multi-step purification to achieve high purity and functional integrity.
TR-FRET Reagents, Assays, and Research Services
Development and execution of High-throughput TR-FRET–based assays using covalent modifiers compatible with terbium/fluorescein and europium/Cy5 donor–acceptor pairs.
Custom Ubiquitin Research
Tailored biochemical studies designed to interrogate the ubiquitin-proteasome system, drug discovery, and drug target validation.
Submit service request with a brief project objective description.
RELAYTR FRET Platform
Fluorescence Resonance Energy Transfer (FRET) is a powerful technique used to detect molecular proximity between a donor and an acceptor fluorophore within a distance of 1–10 nm. In time-resolved FRET (TR-FRET) assays, the long fluorescence lifetime of lanthanides (such as terbium and europium) enables delayed signal detection, effectively eliminating short-lived background fluorescence and, thus, significantly enhancing assay sensitivity and specificity.
Our RELAYTR FRET Platform leverages terbium and europium chelate-based donors paired with fluorescein- and Cy5-based acceptors. These fluorophores can be covalently conjugated to targets via free thiol or amine groups, ensuring robust and reliable performance and versative assay formats for detecting molecular interactions, post-translational modifications, enzyme activity, protein abundance, and more.
Both ready-to-use reagents and customized CRO services are available.
E2 Screening & Profiling
Reagents & Kits
E2 Screening Kit – gel-based Assay
- Identifying E2s for E3s
- Assessing E2 activity
E2 Profiling Kit – RELAYTR FRET Assay
- Monitoring E2-Ub charging (schematic below)
- High sensitivity – detecting 10 nM E2 activity
- High signal to blank (S/B) ratio
- Profiling 23 ubiquitin E2s
- High throughput compatible
CRO Services
- UbE1 & E2 profiling
- Custom high throughput assay development
RELAYTR FRET Assay - E2-Ub Charging
PROTAC & MG Drug Discovery
Reagents and Kits
Terbium, europium, fluorescein or Cy5 labeled ubiquitin, protein G, and anti-6xHis, GST, HA or DYKDDDDK antibody. View all reagents.
CRO Services
- Reagent production – terbium/europium/dye labeling
- E3 ubiquitin ligase activity assay
- E3-substrate interacton assay
- Substrate protein quantification
Proteasome Profiling & Research
Profiling Human 26S Proteasome with Comprehensive Assays
- Peptidase activities
- ATPase activity
- DUB activity
- Degradaton of polyubiquitinated protein
- Binding of polyubiquitin chains
- Gate openning
- Integrity/assembly
Reagents
- Highly purified 19S, 20S and 26S proteasomes
- Fluorogenic substrates – peptidase activities
- Antibodies recognizing proteasomal subunits
- Polyubiquitinated proteins
Kits
- Purification of endogenous 26S proteasome
- Assaying proteasomal activities in cell/tissue extracts
- Assaying proteasome assembly
- Assaying 26S proteasome-mediated protein degradation
DUB Research & Drug Discovery
Reagents
- 30+ recombinant DUBs
- Fluorogenic substrates – Ub-AMC, Ub-Rho110
- Polyubiquitin chains – eight linkages available
- Ubiquitin derivatives – catalytic cysteine labeling
Kits
- Diubiquitin explorer kit
- DUB identification kit
- DUB enzyme set and profiling kit
- RELAYTR FRET deubiquitinase assay kit (see schematic below)
CRO Services
- DUB profiling
- TR-FRET DUB activity assay development
- Recombinant DUB production
Ub Ligase Activity Assay Kit
Key Features
- Detecting Ub ligase autoubiquitination
- High throughput TR-FRET assay enabled with terbium-/fluorescein-ubiquitin
- Excellent signal to blank ratio (S/B) ratio
- High sensitivity and stability
- Fast results in 4 hours
- Ub ligase CHIP included as a positive control
RELAYTR FRET Assay - 25 nM CHIP autoubiquitination
Ready-to-use Ub Ligase Activity Assay Kits
Enriching Ubiquitinated Proteins
Reagents
Kits
- PolySUMO capture Kit
- Free polyUb chain capture kit
- K48 polyUb chain capture kit (Lane 2 below)
- K63 polyUb chain capture kit (Lane 3 below)
- Linear PolyUb chain capture kit (Lane 5 below)
- K29/K33 polyUb chain capture kit
- PolyUb chain capture kit (Lane 4 below)
