Additional Information
| Product Name: | Deubiquitinating Enzyme Inhibitor Profiling Kit I |
|---|---|
| Also Known As: | N/A |
| Catalog No.: | J6110 |
| Size: | 1 Kit |
| Molecular Weight: | N/A |
| Species: | Human |
| Source: | Bacterial or insect cell recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | 85% - 95% based on SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet, M |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Dry ice shipping |
| References: | N/A |
Details
Description
This kit contains 10 deubiquitinating enzymes (DUBs) covering members in four DUB families and the fluorogenic substrate ubiquitin (Ub)-rhodamine 110. Each DUB is provided as 40X stock, and sufficient for 10 X 50 µl (96-well plate) or 25 X 20 µl (384-well plate) reactions. Ub-rhodamine 110 is sufficient for 120 X 50 µl or 300 X 20 µl reactions at 1 µM working concentration. The supplied iodoacetamide can be used as a positive control to inhibit cysteine DUBs at 5 mM working concentration. DUBs have different enzymatic activity, concentration of each DUB is tailored to ensure enzymatic activity determination.
Components
• 10X DUBs .......................................................................................50 µl each
• Ub-rhodamine 110 (50 µM ).............................................................120 µl
• Iodoacetamine (IAA).........................................................................25 mg
• 5X Deubiquitination Buffer II............................................................2X1.25ml
DUB List
1. UCHL1
2. UCHL3
3. OTUB2
4. OTUD2
5. 6xHis-AMSH
6. GST-USP7
7. GST-USP11
8. GST-USP15
9. 6xHis-USP16
10. USP21(195-565)
