USP30(64-502)

USP30(64-502) contains the catalytic domain of USP30 with additional loops being deleted. This variant is stable and highly active compared to the commonly used USP30(57-517) (ref. 4). USP30 deubiquitinates substrates of Parkin including MIRO1, TOMM20, MFN1, and MFN2 on mitochondrial membranes and functions to suppress mitophagy. It prefers cleavage of K6- and K11-linked polyubiquitin chains.

Additional Information

Product Name:

USP30(64-502)

Also Known As:

N/A

Catalog No.:

H7820

Size:

50 µg

Molecular Weight:

37.4 kDa

Species:

Human

Source:

Bacterial recombinant

Stock:

20 mM Tris, 150 mM NaCl, 5 mM TCEP, 10% Glycerol

Concentration:

See tube label

Quality Assurance:

~ 90% by SDS-PAGE

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet， MSDS

NCBI RefSeq:

NM_032663.4

Image(s):

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
(A) Lane 1: Molecular weight markers
Lane 2: 5 µg purified USP30(64-502)
(B) Reactions contained 10 nM USP30(64-502) + 0.5 uM Ub-Rhodamine 110 or Ub-Rhodamine 110 alone in DUB buffer II (cat# H1001). Rhodamine110 fluorescence was measured using a Synergy HTX plate reader with the excitation and emission filter set at 485/20 nm and 528/20 nm, respectively.

Shipping Method:

Dry ice shipping

References:

1. Bingol B, et al. (2014) Nature 510: 370
2. Cunningham CN, et al. (2015) Nat Cell Biol. 17: 160
3. Wang, Y, et al. (2015) Autophagy 11: 595
4. Gersch M, et al. (2017) Nat Struct Mol Biol. 24(11): 920

Details

(A) Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified USP30(64-502)
(B) Dub activity of recombinant USP30(64-502)
Reactions contained 10 nM USP30(64-502) + 500 nM Ub-Rhodamine 110 or Ub-Rhodamine 110 alone in DUB buffer II (cat# H1001). Rhodamine110 fluorescence was measured using a plate reader with the excitation and emission filter set at 485/20 nm and 528/20 nm, respectively.