Additional Information
| Product Name: | Ub-AMC |
|---|---|
| Also Known As: | Ubiquitin-AMC |
| Catalog No.: | M3010 |
| Size: | 50µg |
| Molecular Weight: | 8.7 kDa |
| Species: | Human |
| Source: | Synthetic |
| Stock: | 20 mM Tris, pH 7.1 at 37 0C, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | >95% by SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | Wilkinson KD, et al. (2005) Methods Enzymol. 399, 37-51 |
Details
Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
Images
(Click image to enlarge)
Coomassie-stained SDS-PAGE
5 µg purified Ub-AMC
The reaction contained 2 nM bovine 26S proteasome and 1 mM Ub-AMC. AMC fluorescence was monitored by a plate reader using excitation and emission filter set at 360/40 and 460/30 nm, respectively.
Images
(Click image to enlarge)
Coomassie-stained SDS-PAGE
5 µg purified Ub-AMC
The reaction contained 2 nM bovine 26S proteasome and 1 mM Ub-AMC. AMC fluorescence was monitored by a plate reader using excitation and emission filter set at 360/40 and 460/30 nm, respectively.
