Additional Information
| Product Name: | Rpt3 Rabbit pAb |
|---|---|
| Catalog No.: | Y2222-20 |
| Also Known As: | PSMC4; MIP224; RPT3; S6; TBP-7; TBP7; ATPase 4 |
| Size: | 20 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human Rpt3. |
| Swiss Prot. #: | P43686 |
| Calculated MW: | 47 kDa |
| Detected MW: | 47 kDa |
| Applications: | WB (1:500 - 1:2,000) IF (1:50 - 1:200) IP (not tested) IHC (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Dubiel W, (1994) Biol Chem Hoppe Seyler 375, 237 - 240. 2. Amoroso MR, et al. (2012)Cell Death Differ 19(4), 592 - 604. |
Details
Rpt3 is one the six ATPase subunits of the 26S proteasome. The 26S proteasome is assembled from the 20S proteasome and the 19S regulatory particle that contains 19 different proteins, and possesses polyubiquitin binding, deubiquitination, and ATPase activities. The six ATPase subunits of the 19S regulaotry form a ring in a sequence of Rpt1/Rpt2/Rpt6/Rpt3/Rpt4/Rp5. The ATPase ring interacts with the α ring of the 20S proteasome to form the 26S proteasome that degrades polyubiquitinated proteins.
