Additional Information
| Product Name: | Cy5-Ub-VME |
|---|---|
| Also Known As: | N/A |
| Catalog No.: | M1040 |
| Size: | 25 µg |
| Molecular Weight: | 9.1 kDa |
| Species: | Human |
| Source: | Synthetic |
| Stock: | Product in lyophilized powder form. |
| Concentration: | N/A |
| Quality Assurance: | ≥95% by RP-HPLC |
| Storage: | Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Room temperature shipping |
| References: | 1. Altun et al., (2011) Chem Biol 18, 1401. 2. Misaghi et al., (2005) J. Biol. Chem. 280, 1512. |
Details
Cy5-Ub-VME is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs), which is labeled on the N-terminus with a Cy5 dye (Cy5, Ex 625-650 nm, Em 670 nm). This ubiquitin-based activity probe can be used for activity profiling experiments and labeling of DUBs with a fluorescent dye (Cy5). Cy5 labelling allows for detection of DUB labeling by in-gel fluorescence.
Sample Preparation:
Dissolve the powder in DMSO (e.g. add 1.25 μl DMSO to 25 μg powder to get 20 mg/ml or 2.25 mM DMSO stock) and add this DMSO stock to the required buffer (please note the order of addition). Typical reaction concentrations vary from 0.2 - 2 μM.
Sample Preparation:
Dissolve the powder in DMSO (e.g. add 1.25 μl DMSO to 25 μg powder to get 20 mg/ml or 2.25 mM DMSO stock) and add this DMSO stock to the required buffer (please note the order of addition). Typical reaction concentrations vary from 0.2 - 2 μM.
