Additional Information
| Product Name: | 100X RELAYTR Terbium/Fluorescein Ubiquitin Mix | ||
|---|---|---|---|
| Also Known As: | Tb-Ub; Tb-Ubiquitin; Terbium-Ub; Fluorescein-Ub, FI-Ub | ||
| Catalog No.: | T2501-200 | ||
| Size: | 200 x 20 µl Reactions | ||
| Molecular Weight: | Terbium-Ub (9.5 kDa); Fluorescein-Ub (9 kDa) | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | No | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~ 95% by SDS-PAGE; Validated by RELAYTR ubiquitination assays. | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet | ||
| NCBI RefSeq: | No | ||
| Image(s): | Images:
(Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. https://www.bmglabtech.com/en/tr-fret/ |
Details
Time resolved (TR)-FRET offers a sensitive, low background, stable and homogenous assay for determination of protein ubiquitination. Terbium-ubiquitin and fluorescein-ubiquitin are a pair of modified ubiquitin suitable for TR-FRET assays to assess formation of polyubiquitin chains or multi-monoubiquitination, thereby reporting the activity of E2 and E3 enzymes in the reaction. The concentration and molar ratio of terbium-ubiquitin and fluorescein-ubiquitin in the RELAYTR Terbium/Fluorescein Ubiquitin Mix are optimized to achieve excellent signal-to-blank ratio and validated by multiple E3 autoubiquitination assays. The blank reaction contains UbE1, E2, Terbium/Fluorescein Ubiquitin Mix, and ATP.
Typical protein concentration ranges are: UbE1 (10-30 nM), E2 (50-250 nM) and E3 (10-250 nM). Reaction time is usually 1-3 hours in kinetic or end point assay.
Typical protein concentration ranges are: UbE1 (10-30 nM), E2 (50-250 nM) and E3 (10-250 nM). Reaction time is usually 1-3 hours in kinetic or end point assay.
