Additional Information
| Product Name: | 6xHis-ZnF-UBP | ||
|---|---|---|---|
| Also Known As: | N/A | ||
| Catalog No.: | I1411 | ||
| Size: | 500 µg | ||
| Molecular Weight: | 14.6 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~80% by SDS-PAGE | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | N/A | ||
| Image(s): |
Images:
(Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Hadari T, et al. (1992) J Biol Chem 267, 719 – 727. 2. Reyes-Turcu FE, et al. (2006) Cell 124, 1197 – 1208. 3. Reyes-Turcu FE, et al. (2008) J Biol Chem 283(28), 19581 – 19592. 4. Avvakumov GV, et al. (2012) Biochemistry 51(6), 1188 – 1198. |
Details
Usp5 (IsoT) is a deubiquitinase enzyme that hydrolyzes free polyubiquitin chains. The Usp5 contains two zinc finger domains (ZnF-UBP). This product contains the second ZnF-UBP domain (amino acid 163 – 291). The ZnF-UBP domains bind free ubiquitin chains’ diglycine carboxyl tail, which defines its specificity in binding of free polyubiquitin chains.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-ZnF-UBP |
