Additional Information
| Product Name: | 6xHis-Nedd8 |
|---|---|
| Also Known As: | NEDD-8, NEDD 8 |
| Catalog No.: | E4100 |
| Size: | 500 µg |
| Molecular Weight: | 9 kDa |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | NM_006156 |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | 1. Kumar S, et al. (1993) Biochem Biophys Res Commun 195(1), 393 – 399. 2. Whitby FG, et al. (1998) J Biol Chem 273(52), 34983 – 34991. 3. Soucy TA, et al. (2010) Genes Cancer 1(7), 708 – 7016. |
Details
Nedd8 is an 81 amino acid Ub-like protein. It is conjugated to protein substrates in much the same way as Ub, using the E1, E2, E3 cascade. The specific E1 used is a heterodimer of APPBP1/UBA3. Two E2s have been discovered to follow called Ubc12 and Ube2F. Neddylation causes conformational change of the Cullin protein in the E3 called cullin-RING ligases (CRLs), which enhances the E3 Ub ligase activity of CRLs.
