Additional Information
| Product Name: | Fluorescein-Protein G |
|---|---|
| Also Known As: | Fluorescein labeled protein G |
| Catalog No.: | T7010-200 |
| Size: | 200 µg |
| Molecular Weight: | 22kDa |
| Species: | G Streptococci |
| Source: | Recombinant protein G |
| Stock: | 10 mM PBS pH 7.2, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | > 95% purity based on SDS-PAGE. |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet |
| NCBI RefSeq: | No |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | 1. https://www.bmglabtech.com/en/tr-fret/ |
Details
Fluorescein is covalently conjugated on lysines of recombinant protein G. The molar ratio between fluorescein and protein G in different lots is ~1 - 2. Protein G has higher affinity than protein A in binding of mouse, human, rat, goat and cow IgG.
Fluorescein-protein G recognizes various antibodies and can serve as the acceptor in TR-FRET assays that offer a sensitive tool in monitoring molecular proximity, post-translational modifications, enzyme activity and protein abundance. The donor is usually a terbium-based chelator.
Fluorescein-protein G can also be used in varous fluorescent assays, including fluorescent western blotting and immunofluorescence microscopy.
