Fluorescein-Anti-GST Mouse mAb

Fluorescein (6-FAM) is covalently conjugated on anti-GST mouse mAb (clone #: GST.B6), and purified by gel filtration. Usually, the molar ratio of fluorescein to antibody is at the range of 3-10.

In TR-FRET assays, a typical concentration of fluorescein labeled antibody is 0.8-1.2-fold of the concentration of the target protein. User should optimize the assay to achieve a desirable signal-to-background ratio, including the concentration and ratio of fluorescein or terbium labeled antibody, IgG or proteins corresponding to your specific assays.

Additional Information

Product Name:

Also Known As:

Fluorescein-Anti-GST Antibody; FL-Anti-GST

Catalog No.:

T6020-50

Size:

50 µg

Molecular Weight:

150 kDa

Species:

Mouse

Source:

Stock:

10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide)

Concentration:

See tube label

Quality Assurance:

Validated by RELAY^TR assay

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet

NCBI RefSeq:

Image(s):

Images:
(Click image to enlarge)

A. Schematic of the RELAYTR GST-UbE3A Autoubiquitination Assay using fluorescein-Anti-GST antibody and terbium-Ubiquitin.
B. The reaction contained 10 nM UbE1, 100 nM UbE2D4, 12.5 nM GST-UbE3A, ubiquitin and terbium-ubiquitin. After 1.5 hr reaction under room temperature, fluorescein-Anti-GST antibody was added. TR-FRET signal was monitored by an end point mode. Reactions with ATP were positive signal (S), and without ATP were background (B). The signal-to-background ratio was calculated by using the formula of (S-B)/B. Data were from triplicates as mean ± S.D.

Shipping Method:

Wet ice shipping

References:

1. https://www.bmglabtech.com/en/tr-fret/

Details

Fluorescein (6-FAM) is covalently conjugated on anti-GST mouse mAb (clone #: GST.B6), and purified by gel filtration. Usually, the molar ratio of fluorescein to antibody is at the range of 3-10.
In TR-FRET assays, a typical concentration of fluorescein labeled antibody is 0.8-1.2-fold of the concentration of the target protein. User should optimize the assay to achieve a desirable signal-to-background ratio, including the concentration and ratio of fluorescein or terbium labeled antibody, IgG or proteins corresponding to your specific assays.