RELAYTR MDM2 Activity Assay Kit

RELAYTR MDM2 Activity Assay Kit utilizes the TR-FRET technology to measure the ubiquitin ligase activity of MDM2 by detecting its autoubiquitination. The kit is based on the terbium/fluorescein pair for FRET detection. It is easy to use, sensitive, and stable under room temperature operation.


Implications

1) Screening inhibitors/of MDM2.

2) Profiling ubiquitin ligases.

3) Assessing the ubiquitin ligase activity of MDM2.



Additional Information

Product Name: RELAYTR MDM2 Activity Assay Kit
Also Known As: TR-FRET MDM2 Ubiquitin Ligase Activity Assay Kit
Catalog No.: T4060
Size: 400 x 20 µl Reactions
Molecular Weight: No
Species: Human
Source: Recombinant proteins
Stock: No
Concentration: See tube label
Quality Assurance: Validated by RELAYTR ubiquitination assays.
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: No
Image(s): Images:
(Click image to enlarge)

RS is the FRET ratio of positive reaction; RB is the FRET ratio of blank reaction (no E3 compared to positive reaction). Data (mean ± S.D. of four reactions) were obtained from 50 nM 6xHIS-MDM2 autoubiquitination using the kit. Time 0 is ~5 min after ATP addition.

Shipping Method: Dry ice shipping
References: 1. https://www.bmglabtech.com/en/tr-fret/

Details

RELAYTR MDM2 Activity Assay Kit utilizes the TR-FRET technology to measure the ubiquitin ligase activity of MDM2 by detecting its autoubiquitination. The kit is based on the terbium/fluorescein pair for FRET detection. It is easy to use, sensitive, and stable under room temperature operation.
Implications
1) Screening inhibitors of MDM2.
2) Profiling ubiquitin ligases.
3) Assessing the ubiquitin ligase activity of MDM2.