Additional Information
| Product Name: | PLPro Deubiquitinase Activity Assay Kit II |
|---|---|
| Also Known As: | SARS-CoV-2 Papain-like Protease Deubiquitinase Activity Assay Kit |
| Catalog No.: | J7651 |
| Size: | 1 Kit (1200 assays) |
| Molecular Weight: | N/A |
| Species: | N/A |
| Source: | N/A |
| Stock: | N/A |
| Concentration: | See tube label |
| Quality Assurance: | Enzymatic activity validated |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | J7651 image Inhibition of PLPro’s proteinase or DUB activity by GRL-0617. Reactions contained 20µM Dabcyl–FRLKGGAPIKGV–Edans and 150nM PLPro (squire symbols), or 0.75µM Ub-Rhodamine110 and 60nM PLPro (circle symbols). GRL-0617 was added as a series of 2X titration in reactions. PLPro activity without GRL-0617 was referenced as 100% activity. % of PLPro activity was plotted vs. Log[µM] of GRL-0617. Data were shown as mean ± SD of triplicates. |
| Shipping Method: | Dry ice shipping |
| References: | N/A |
Details
The kit measures the deubiquitinase activity of SARS-CoV-2 papain-like protease (PLPro), can be used for screening/profiling PLPro inhibitors. Kit I is sufficient for 120 X 50µl reactions; Kit II is sufficient for 1,200 X 50µl reactioons. Optimized for rapidly and quantitatively measuring PLPro activity under room temperature. .
Key Features:
• Optimized for room temperature operation, particularly useful for HTS assays
• Rapid and quantitative result within 2-3 hours
• Positive PLPro inhibitor GRL-0617 included
• Large excess amount of buffer provided
Components included in the kit
10X PLPro (Enzyme)................................................... 3µM, 1.2ml
10X Ubiquitin-Rhodamine 110 (Substrate).................. 37.5µM, 1.2ml
100X GRL-0617 (Inhibitor) ...................................... 10mM in DMSO, 20µl
5X Assay Buffer........................................................... 24ml
