K48 Ub4-UbcH10

K48-linked Ub4 is covalently conjugated on lysine residues of UbcH10, with the majority of the product containing one Ub4. This ubiquitin chain length defined substrate is well-suited for in vitro assays evaluating deubiquitination, polyubiquitinated substrate binding, and proteasomal degradation.

Additional Information

Product Name: K48 Ub4-UbcH10
Also Known As: Ub4 (k48-linked)-UbcH10; K48 Ub4-UbE2C
Catalog No.: G7100
Size: 20 µg
Molecular Weight: N/A
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 80 mM imidazole, 10% Glycerol
Concentration: See tube label
Quality Assurance: >80% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet, MSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

A. 3 µg K48 Ub4-UbcH10 in SDS-PAGE, Coomassie stained.
B.Degradation of 100 nM K48-Ub4-UbcH10 by 25 nM human 26S proteasome (Catalog# A1100). Detected by immunoblotting of the 6xHIS tag present in both UbcH10 and the proteaosmal subunit Rpn11. 100 µM epoxomicin was added to inhibit 26S proteasome as a control.

Shipping Method: Dry ice shipping
References: N/A

Details

K48-linked Ub4 is covalently conjugated on lysine residues of UbcH10, with the majority of the product containing one Ub4. This ubiquitin chain length defined substrate is well-suited for in vitro assays evaluating deubiquitination, polyubiquitinated substrate binding, and proteasomal degradation.