Additional Information
| Product Name: | Dabcyl–FRLKGGAPIKGV–Edans | ||
|---|---|---|---|
| Also Known As: | Dabcyl–FRLKGGAPIKGV–Edans , Dabcyl–FRLKGGAPIKGV–Edans.TFA, Dabcyl–Phe-Arg-Leu-Lys-Gly-Gly-Ala-Pro-Ile-Lys-Gly-Val-Edans, Dabcyl–Phe-Arg-Leu-Lys-Gly-Gly-Ala-Pro-Ile-Lys-Gly-Val-Edans.TFA | ||
| Catalog No.: | V1020-25 | ||
| Size: | 25 mg | ||
| Molecular Weight: | 1742.1 Da | ||
| Species: | N/A | ||
| Source: | Synthetic | ||
| Stock: | Powder | ||
| Concentration: | N/A | ||
| Quality Assurance: | >95% by HPLC | ||
| Storage: | Eligible for room temperature shipping. Store at -20°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO. Protect from light. | ||
| PDF Data Sheet: | Download PDF datasheet, MSDS | ||
| NCBI RefSeq: | N/A | ||
| Image(s): | Click image to enlarge)
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| Shipping Method: | Room temperature shipping | ||
| References: | No |
Details
Dabcyl–FRLKGGAPIKGV–Edans is an internally quenched fluorogenic substrate for the SARS-CoV or MERS-CoV family Papain-like protease (PLPro or PLP) that recognizes a LXGG consensus cleavage sites. Upon PLP-mediated cleavage between glycine and alanine, the resulting APIKGV-Edans fluorescence can be monitored at the excitation/emission wavelengths of 355/538 nm, respectively. In addition, PLP has deubiquitinating and deISG15ylation activities.
The working concentration is 20-50 µM.
