GST-MURF2

MURF2, also known as TRIM55 (Tripartite motif-containing protein 55), functions as a striated muscle-specific RING-type E3 ubiquitin ligase. It acts as a critical signaling molecule and cytoskeletal adaptor involved in cardiac and skeletal muscle development, sarcomere assembly, and protein turnover.

Additional Information

Product Name:

GST-MURF2

Also Known As:

RNF29, TRIM55, Muscle-specific RING finger protein 2, MuRF2

Catalog No.:

K2010

Size:

50 µg

Molecular Weight:

86.6 kDa (with GST tag)

Species:

Human

Source:

Bacterial recombinant

Stock:

20 mM Tris, pH7.6 at 4 ⁰C, 150 mM NaCl, 2 mM βME, 10% Glycerol, 0.01% Tween-20.

Concentration:

See tube label

Quality Assurance:

~75% purity based on SDS-PAGE. Validated E3 ubiquitin ligase activity.

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet

NCBI RefSeq:

Image(s):

(Click image to enlarge)

A. 3 ug GST-MURF2 separated in an 8-16% Tris-glycine gel (Coomassie-stained).
B. GST-MURF2 autoubiquitination. The reaction contained 50 nM UbE1, 1 µM UbE2D4, 0.5 µM GST-MURF2, and 50 µM ubiquitin. Proteins were separated in an 8-16% Tris-glycine gel, visualized by Coomassie-staining.
C. GST-MURF2 autoubiquitination detected by RELAYTR FRET assay (see product # T4100). Blue/orange/green lines represent results with 25 nM, 12.5 nM and 6.25 nM GST-MURF2, respectively.

Shipping Method:

Dry ice shipping

References:

1. McElhinny A. S, et al. (2004) J cell Sci. 117, 3175-88

GST-MURF2

Additional Information

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