GST-3CLPro

Maturation of SARS-CoV-2 virus requires two proteases, 3C-like protease (3CLPro) and Papain-like protease (PLPro), to cleave the large polyproteins 1a and 1ab to generate functional proteins. which is essential for genome replication and Coronavirus virion production. Both proteases are encoded by the polyproteins. 3CLPro is a cysteine protease and forms a homodimer. 3CLPro is the drug target of PF-07321332, a main component of the oral anti-Covid-19 drug Paxlovid. PLPro is also a cysteine protease that cleaves polyproteins and has deubiquitinating and deISG15ylating activities. PLPro is also a potential anti-Covid-19 drug target.

The recombinant SARS-CoV-2 3C-like protease contains the catalytic domain from Ser3264-Gln3569.

Additional Information

Product Name:

GST-3CLPro

Also Known As:

3CLP, 3CLPro, Main protease, Mpro, Sars Covid-19 3CLPro

Catalog No.:

V2010

Size:

50 µg

Molecular Weight:

60.2 kDa

Species:

SARS-Cov-2

Source:

Bacterial recombinant

Stock:

20 mM Tris, pH7.6 at 4 0C, 150 mM NaCl, 4mM TCEP, 10% Glycerol and 0.01% Tween-20

Concentration:

See tube label

Quality Assurance:

> 90% purity based on SDS-PAGE.

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF Datasheet， MSDS

NCBI RefSeq:

N/A

Image(s):

(Click image to enlarge)

A. Coomassie stained SDS-PAGE of 5 ug GST-3CLPro.
B. Characterization of GST-3CLPro activity.
Solid cirlce: 20µM Dabcyl-KTSAVLGSGFRKME-Edans (substrate, catalog# V1010).
Triangle : Substrate + 100nM GST-3CLPro (catalog# V2010)+ 10µM PF-07321332 (catalog# F1000).
Square: Substrate + 100nM GST-3CLPro.

Shipping Method:

Dry ice shipping

References:

1. Lucast LJ.,et al. (2001) Biotechniques 30, 544.

Details

Maturation of SARS-CoV-2 virus requires two proteases, 3C-like protease (3CLPro) and Papain-like protease (PLPro), to cleave the large polyproteins 1a and 1ab to generate functional proteins. which is essential for genome replication and Coronavirus virion production. Both proteases are encoded by the polyproteins. 3CLPro is a cysteine protease and forms a homodimer. 3CLPro is the drug target of PF-07321332, a main component of the oral anti-Covid-19 drug Paxlovid. PLPro is also a cysteine protease that cleaves polyproteins and has deubiquitinating and deISG15ylating activities. PLPro is also a potential anti-Covid-19 drug target.
The recombinant SARS-CoV-2 3C-like protease contains the catalytic domain from Ser3264-Gln3569.