Additional Information
| Product Name: | Rpn11 Rabbit pAb |
|---|---|
| Catalog No.: | Y2402-20 |
| Also Known As: | PSMD14; PAD1; POH1; RPN11 |
| Size: | 20 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-300 of human Rpn11. |
| Swiss Prot. #: | O00487 |
| Calculated MW: | 35 kDa |
| Detected MW: | 35 kDa |
| Applications: | WB (1:500 - 1:2,000) IF (1:50 - 1:100) IP (not tested) IHC (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Spataro V, et al. (1997) J Biol Chem 272, 30470 - 30475. 2. Yao T and Cohen RE, (2002) Nature 419, 403 - 407. |
Details
Rpn11 is a subunit of the 19S regulatory particle (PA700). It is a metalloprotease that catalyzes en bloc deubiquitination to remove a whole polyub chain from a substrate protein. The 26S proteaosme is composed of 33 different proteins and responsible for degrading polyubiquitinated proteins in a ATP hydrolysis-dependent manner. The 19S regulatory particle can bind polyubiqutin chains to recruit substrates, deubiquitinate to recycle ubiquitin, unfold substrate proteins under ATP hydrolysis, and translocate unfolded proteins to the 20S proteasome for hydrolysis.
