Additional Information
| Product Name: | Caspase-3 Rabbit pAb [KO Validated] |
|---|---|
| Catalog No.: | Z1632-20 |
| Also Known As: | CASP3; CPP32; CPP32B; SCA-1; Active Caspase 3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3 |
| Size: | 20 µl |
| Concentration: | See labels on tube |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase-3 (NP_004337.2) |
| Swiss Prot. #: | P42574 |
| Calculated MW: | 31 kDa |
| Detected MW: | 17 kDa/35 kDa |
| Applications: | WB (1:500 - 1:2,000) IHC (1:50 - 1:200) IP (not tested) IF (not tested) Note: Antibody dilution should be optimized by users. |
| Purification: | Protein A or G affinity purification |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| Image(s): | (Click image to enlarge)  |
| Shipping Method: | Wet ice shipping |
| References: | 1. Fernandes - Alnemri T, et al. (1994) J Biol Chem 269, 30761 - 30764. 2. Nicholson DW, et al. (1995) Nature 376, 37 - 43. |
Details
Caspase-3 is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments.
