Additional Information
| Product Name: | Anti-beta-Tubulin |
|---|---|
| Also Known As: | No |
| Catalog No.: | Y1060 |
| Size: | 25 µg |
| Molecular Weight: | ~55/25 kDa |
| Species: | Human |
| Source: | Immunogen: β-Tubulin N-terminal peptide-KLH conjugates |
| Stock: | 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
| Concentration: | 1 µg/µl |
| Quality Assurance: | Guaranteed for detecting endogenous β-Tubulin in 20 µg cell or tissue lysates |
| Storage: | Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge)
 Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)v  Figure 2. 1:4000 (0.25 μg/ml) Ab dilution used in WB of 10 μg/lane (1), 5 μg/lane (2), 2.5 μg/lane (3) and 1.25 μg/lane (4) mouse brain tissue lysates |
| Shipping Method: | Wet ice shipping |
| References: | N/A |
Details
Specificity
Recognizes native and denatured forms of β-Tubulin (~50kDa)
Isotype
Mouse IgG2a
Clone
BT7R
Purification
Protein A affinity chromatography from mouse ascites fluid
Applications
Dot, ELISA, IS, WB
Cross Reactivity
β-Tubulin from human, monkey, mouse, rat, rabbit, chicken. β-Tubulin from other species may also be detectable
Working Conditions
WB (with ECL): 1:1000-5000 dilution
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test
Images
(Click image to enlarge)
Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)v
Figure 2. 1:4000 (0.25 μg/ml) Ab dilution used in WB of 10 μg/lane (1), 5 μg/lane (2), 2.5 μg/lane (3) and 1.25 μg/lane (4) mouse brain tissue lysates
Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)v
Figure 2. 1:4000 (0.25 μg/ml) Ab dilution used in WB of 10 μg/lane (1), 5 μg/lane (2), 2.5 μg/lane (3) and 1.25 μg/lane (4) mouse brain tissue lysates
