Additional Information
| Product Name: | Anti-MBP |
|---|---|
| Also Known As: | Anti-Maltose-binding Protein Mouse Monoclonal Antibody |
| Catalog No.: | Y1001 |
| Size: | 100 µg |
| Molecular Weight: | ~55/25 kDa |
| Species: | N/A |
| Source: | Immunogen: recombinant MBP protein |
| Stock: | 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
| Concentration: | 1 µg/µl |
| Quality Assurance: | WB (with ECL): 5 - 50 ng of purified MBP or MBP fusion proteins |
| Storage: | Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge)
 1:2000 (0.5 μg/ml) Ab dilution probed recombinant MBP protein at 50ng (lane 1), 24 ng (lane 2), 12ng (lane 3), 6ng (lane 4) and 3ng (lane 5). |
| Shipping Method: | Wet ice shipping |
| References: | N/A |
Details
Specificity
Recognizes MBP or MBP fusion proteins
Isotype
Mouse IgG2b
Clone
MBP61R
Purification
Protein A affinity chromatography from mouse ascites fluid
Applications
Dot, ELISA, IS, IP, WBv
Sensitivity
WB (with ECL): 5 - 50 ng of purified MBP or MBP fusion proteins
Working Conditions
WB (with ECL) 1:1000 dilution
IS: 1:500 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Images
(Click image to enlarge)
1:2000 (0.5 μg/ml) Ab dilution probed against recombinant MBP protein at 50ng (lane 1), 24 ng (lane 2), 12ng (lane 3), 6ng (lane 4) and 3ng (lane 5).
1:2000 (0.5 μg/ml) Ab dilution probed against recombinant MBP protein at 50ng (lane 1), 24 ng (lane 2), 12ng (lane 3), 6ng (lane 4) and 3ng (lane 5).
