Additional Information
| Product Name: | Anti-GFP |
|---|---|
| Also Known As: | Anti-GFP (GF28R) Mouse Monoclonal Antibody |
| Catalog No.: | Y1030 |
| Size: | 25 µg |
| Molecular Weight: | ~55/25 kDa |
| Species: | N/A |
| Source: | Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates |
| Stock: | 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
| Concentration: | 1 µg/µl |
| Quality Assurance: | WB (with ECL): 1-10 ng of purified GFP or GFP fusion proteins |
| Storage: | Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge)
 1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used |
| Shipping Method: | Wet ice shipping |
| References: | N/A |
Details
Specificity
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Isotype
Mouse IgG2b
Clone
GF28R
Purification
Protein A affinity chromatography from mouse ascites fluid
Applications
Dot, ELISA, IP, IS, WB
Working Conditions
WB (with ECL): 1:1000-3000 dilution
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Images
(Click image to enlarge)
1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used
1:1000 (1 μg/ml) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (Lane 1), 1μg (Lane 2) and 10μg (Lane 3) of cell lysates used
